Effects of human tissue kallikrein gene transfer on the migration of vascular smooth muscule cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 746-749, 2010.
Article
in Zh
| WPRIM
| ID: wpr-355028
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of adenovirus-mediated human tissue kallikrein (Ad-hKLK1) gene transfer on platelet-derived growth factor-BB (PDGF-BB)-induced migration of vascular smooth muscle cells from spontaneously hypertensive rats (VSMC(SHR)).</p><p><b>METHODS</b>A bicistronic recombinant adenovirus vector (Ad-hKLK1) carrying the target hKLK1 gene and the reporter gene EGFP was constructed. VSMCs isolated from the thoracic aorta of male SHR were passaged, and the quiescent VSMC(SHR) in passages 3-6 seeded in 6-well plates were treated with Ad-hKLK1 and control virus. Human PDGF-BB or icatibant Hoe140, a BK B2 antagonistat, was used as the chemoattractant and placed in the bottom chamber of the Boyden chamber. The mRNA expressions of bradykinin B1 receptor and B2 receptor were detected by RT-PCR in VSMC(SHR).</p><p><b>RESULTS</b>hKLK1 gene transfer significantly inhibited PDGF-BB-induced migration of VSMC(SHR), with the peak inhibition rate of 34.6% (P<0.001). PDGF-BB significantly increased the mRNA expression of B2 receptor but not B1 receptor in VSMC(SHR).</p><p><b>CONCLUSIONS</b>hKLK1 gene transfer can inhibit the migration of VSMC(SHR) induced by PDGF-BB, and the inhibitory effects may be not mediated by bradykinin B2 receptor.</p>
Full text:
1
Index:
WPRIM
Main subject:
Aorta, Thoracic
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Pathology
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Pharmacology
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Rats, Inbred SHR
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Recombinant Proteins
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Platelet-Derived Growth Factor
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Cell Movement
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Cells, Cultured
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Adenoviridae
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Gene Transfer Techniques
Limits:
Animals
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Humans
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Male
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article