Human soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin inhibits phagocytosis of Staphylococcus aureus by immature dendritic cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 544-548, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-355331
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (sDC-SIGN) on the phagocytosis of Staphylococcus aureus (S. aureus) by immature dendritic cells (imDCs).</p><p><b>METHODS</b>Flow cytometry was employed to examine the effect of sDC-SIGN on the phagocytosis of S. aureus by imDCs. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the binging of sDC-SIGN to S. aureus, lipoteichoic acid (LTA) and lipopolysaccharides (LPS) and investigate the effect of the ligands mannan and LTA and anti-DC-SIGN antibodies 1C6 and 4H3 on the binging of sDC-SIGN to S. aureus.</p><p><b>RESULTS</b>sDC-SIGN inhibited the phagocytosis of S. aureus by imDCs. sDC-SIGN bound to S. aureus in a Ca(2+)-dependent manner. sDC-SIGN concentration-dependently bound to LTA, but not to LTA, and the binging of sDC-SIGN to S. aureus was blocked by mannan, LTA, 1C6 and 4H3.</p><p><b>CONCLUSION</b>sDC-SIGN preferentially binds to the carbohydrate constituents on S. aureus to affect the binding between membrane-bound DC-SIGN and S. aureus, thus suppressing the phagocytosis of S. aureus by imDCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phagocytosis
/
Staphylococcus aureus
/
Teichoic Acids
/
Dendritic Cells
/
Cell Adhesion Molecules
/
Lipopolysaccharides
/
Receptors, Cell Surface
/
Cell Biology
/
Lectins, C-Type
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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