Human bone marrow mesenchymal stem cells cocultured with semi-permeable membrane separated neonatal rat ventricular myocytes differentiated into cardiomyocyte phenotype / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 651-654, 2008.
Article
in Zh
| WPRIM
| ID: wpr-355920
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the ability of human bone marrow mesenchymal stem cells (hBMSCs), cocultured with semi-permeable membrane separated neonatal rat ventricular myocytes, to differentiate into cardiomyocytes.</p><p><b>METHODS</b>hBMSCs were isolated and purified by density gradient centrifugation and adherence screening method. Cells were expanded as undifferentiated cells in culture for more than 3 passages and their phenotypes were identified with flow cytometer. hBMSCs were cocultured with neonatal rat ventricular myocytes in a rate of 110 separated by semi-permeable membrane. GATA4 mRNA was detected by RT-PCR; Immunocytochemistry, and Immunostaining were used to detect sarcomeric alpha-actinin, desmin, cTnT, and cTnI protein level.</p><p><b>RESULTS</b>CD29 (98.64% +/- 0.80%) and CD44 (96.70% +/- 1.50%) were the major surface markers of hBMSCs. After coculturing with semi-permeable membrane separated neonatal rat ventricular myocytes, the first contraction of single cells was noted at day 7 and GATA4 expression was detected on these cells by RT-PCR after 1 to 3 weeks coculture. Desmin, sarcomeric alpha-actinin, cTnI and cTnT could be detected by immunocytochemistry and immunostaining on some of these cells.</p><p><b>CONCLUSION</b>hBMSCs possess the potential to differentiate into myocardial cell phenotype in the cardiac microenvironment. Direct contact with cardiomyocytes was not necessary required for hBMSCs differentiation.</p>
Full text:
1
Index:
WPRIM
Main subject:
Bone Marrow Cells
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Cell Differentiation
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Cells, Cultured
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Rats, Sprague-Dawley
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Coculture Techniques
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Cell Culture Techniques
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Cell Biology
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Myocytes, Cardiac
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Mesenchymal Stem Cells
Limits:
Animals
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Humans
Language:
Zh
Journal:
Chinese Journal of Cardiology
Year:
2008
Type:
Article