Genome amplification and sequence analysis of human bocavirus 2 / 病毒学报
Chinese Journal of Virology
;
(6): 257-262, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-356606
ABSTRACT
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Virology
/
RNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Gene Amplification
/
Classification
/
Genome, Viral
/
Parvoviridae Infections
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article
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