Determination of scopolamine and atropine in Flos Daturae by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1065-1067, 2006.
Article
in Zh
| WPRIM
| ID: wpr-356699
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC.</p><p><b>METHOD</b>The two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature.</p><p><b>RESULT</b>The mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine.</p><p><b>CONCLUSION</b>This method was simple, accurate and sensitive.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
/
Atropine
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Scopolamine
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
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Datura
/
Flowers
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Methods
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article