Estrogenicity of adzuki bean Phaseolus angularis wight. and its effect on proesterone receptor level in human breast cancer MCF-7 cells / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1261-1265, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-356729
ABSTRACT
<p><b>OBJECTIVE</b>To explore the estrogenic activity of ethanol extract from adzuki bean Phaseolus angularis and its effect on progesterone receptor (PR) level of human breast cancer MCF-7 cells.</p><p><b>METHOD</b>The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of adzuki bean extract. And its effect on PR mRNA and protein were addressed using RT-PCR measurements and western blotting analysis respectively in which pure estrogen receptor antagonist ICI 182,780 was employed as the tool.</p><p><b>RESULT</b>The estrogenic activity of adzuki bean extract at various concentrations (10-200 microg x mL(-1)), expressed as proliferative effect (PE) relative to that of solvent control, was examined. The results indicated that the extract of adzuki bean was able to induce MCF-7 cell growth with a maximum at 100 microg x mL(-1). The results of RT-PCR and western blotting showed that PR mRNA and protein could be significantly induced by adzuki bean extract in MCF-7 cells. Moreover, the specific estrogen receptor antagonist ICI 182,780 could block these reactions.</p><p><b>CONCLUSION</b>Ethanol extract of adzuki bean has the estrogen-like activities through the estrogen response pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Plants, Medicinal
/
Seeds
/
Breast Neoplasms
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Receptors, Progesterone
/
Chemistry
/
Phaseolus
Limits:
Female
/
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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