Therapeutic effect of enhancer of Zeste homolog 2 inhibitor GSK343 on periodontitis by regulating macrophage differentiation / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 264-268, 2017.
Article
in Zh
| WPRIM
| ID: wpr-357524
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the therapeutic effect of enhancer of Zeste homolog 2 (EZH2) inhibitor GSK343 on periodontitis by regulating microphage differentiation.</p><p><b>METHODS</b>Macrophage RAW264.7 cells were divided into the blank (A group), control (B group), lipopolysaccharide (LPS) stimulation (C group), and LPS+GSK343 (D group) groups. Phenotype transformations was determined through Western blot analysis and enzyme-linked immunosorbent assay by detecting the differentiation of phenotypic biological markers, including tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), interleukin-10 (IL-10), and Arginase-1 (Arg-1). Metergasis was identified by performing a phagocytosis test on Escherichia coli (E. coli).</p><p><b>RESULTS</b>Macrophage RAW264.7 cells produced classical phenotypic biomarkers (M1) TNF-α and iNOS under LPS stimulation. The expression levels of IL-10 and Arg-1 increased after adding GSK343 into the culture medium. GSK343 also induced the conversion of M1 macrophages into M2 macrophages. Macrophage RAW264.7 cells exerted a phagocytic effect on E. coli, and this effect was enhanced after adding LPS into the culture medium. GSK343 regulated the macrophage RAW264.7 phagocytosis of E. coli.</p><p><b>CONCLUSIONS</b>GSK343 possibly participates in the regulation of macrophage differentiation and, consequently, in the latent treatment of periodontitis.</p>
Key words
Full text:
1
Index:
WPRIM
Main subject:
Periodontitis
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Phagocytosis
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Pharmacology
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Arginase
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Pyridones
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Enzyme-Linked Immunosorbent Assay
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Cell Differentiation
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Lipopolysaccharides
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Tumor Necrosis Factor-alpha
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Interleukin-10
Type of study:
Prognostic_studies
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2017
Type:
Article