Detection of epidermal growth factor receptor gene mutations and its clinical significance in non-small cell lung cancers / 中华病理学杂志
Chinese Journal of Pathology
; (12): 671-674, 2011.
Article
in Zh
| WPRIM
| ID: wpr-358267
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the detection technology and its clinical significance of the EGFR gene mutation in non-small cell lung cancer.</p><p><b>METHODS</b>DNA direct sequencing methods by PCR amplification were used to detect EGFR gene exons 18-21 mutation and to analyze its clinical pathological significance in 192 patients with non-small cell lung cancer.</p><p><b>RESULTS</b>64 of the 192 cases presented with EGFR gene tyrosine kinase binding domain mutation (64/192, 33.3%), of which exon 19 deletion mutation rate was 60.9% (39/64), exon 21 alternative mutation rate was 39.1% (25/64), but exons 18 and 20 mutation was not found in this group of patients. EGFR gene mutation rate was 58.5%(24/41) in lung adenocarcinoma associated with bronchioloalveolar carcinoma differentiation, which was significantly higher than that of ordinary adenocarcinoma (37.9%, 33/87), squamous cell carcinoma (7.5%, 4/53), large cell carcinoma (1/5) and adenosquamous carcinoma (2/6, P<0.05). EGFR gene mutation rates in male patients (20.9%, 24/115), were significantly higher than in the females (51.9%, 40/77; P<0.01); non-smokers (50.0%, 57/114), significantly higher than that of smokers (9.0%, 7/78; P<0.01).</p><p><b>CONCLUSIONS</b>DNA direct sequencing method by PCR amplification is stable and reliable in detection of EGFR gene mutation in non-small cell lung cancer. It might provide a scientific basis for targeted therapy.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Adenocarcinoma
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Smoking
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Sex Factors
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Polymerase Chain Reaction
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Exons
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Sequence Analysis, DNA
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Carcinoma, Non-Small-Cell Lung
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Adenocarcinoma, Bronchiolo-Alveolar
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Genes, erbB-1
Type of study:
Diagnostic_studies
Limits:
Adult
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Aged
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2011
Type:
Article