Association of thymidylate synthase polymorphism with esophageal squamous-cell carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 475-477, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-358599
ABSTRACT
<p><b>OBJECTIVE</b>To explore the influence of 5'UTR tandem repeat and 3'UTR 6 bp deletion polymorphism of thymidylate synthase (TS) gene on the development and lymphatic metastases of esophageal squamous-cell carcinoma (ESCC).</p><p><b>METHODS</b>Peripheral leucocyte DNA was extracted from 232 ESCC patients and 348 age- and sex-matched healthy controls. TS 5'UTR and 3'UTR genotyping in all study subjects was performed by PCR fragment analysis and PCR-RFLP analysis, respectively.</p><p><b>RESULTS</b>The distribution of TS 5'UTR and 3'UTR variants in ESCC patients was not significantly different from that in healthy controls. However, individuals with 6 bp+/6 bp+ and 3R/3R genotypes significantly reduced the risk to ESCC development compared to those with other genotype combinations (adjusted OR = 0.32, 95% CI = 0.08-0.92). In addition, the frequency of 2R/3R genotype in ESCC patients with lymphatic metastases (40%) was significantly higher than that in lymph node negative cases (14.7%) (chi(2) = 10.11, P = 0.001). Compared to 3R/3R genotype, the 2R/3R genotype significantly increased the risk of lymphatic metastases in ESCC (adjusted OR = 3.68, 95% CI = 1.54-8.93).</p><p><b>CONCLUSION</b>The genotyping of TS 5'UTR and 3'UTR polymorphisms might be used as a stratification maker for predicting susceptibility to ESCC. The TS 5'UTR 2R/3R genotype might be a candidate molecular marker to predict the potential of lymphatic metastases in ESCC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Polymorphism, Genetic
/
Thymidylate Synthase
/
Esophageal Neoplasms
/
Carcinoma, Squamous Cell
/
Biomarkers, Tumor
/
Tandem Repeat Sequences
/
Genetics
/
Genotype
/
Lymphatic Metastasis
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2005
Type:
Article
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