Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-alpha release of endothelial cells / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 656-661, 2008.
Article
in En
| WPRIM
| ID: wpr-359368
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of down-regulated tryptase expression in mast cells on the synthesis and release of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) of vascular endothelial cells.</p><p><b>METHODS</b>Tryptase-siRNA (small-interfering RNA) vector was constructed to inhibit tryptase expression in P815 cells. The medium of P815 cells treated by the tryptase-siRNA (RNAi-P815 group) or pure vector (P815 group) was collected and used to culture bEnd.3 cells. The messenger RNAs (mRNAs) of IL-6 and TNF-alpha in bEnd.3 cells and their protein levels in the medium were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively.</p><p><b>RESULTS</b>IL-6 and TNF-alpha mRNAs in bEnd.3 cells cultured in RNAi-P815-conditioned medium decreased significantly compared to those in P815-conditioned medium. Consistently, IL-6 and TNF-alpha protein levels in the medium of bEnd.3 of RNAi-P815 group were lower than those of P815 group.</p><p><b>CONCLUSION</b>Reduced tryptase expression significantly inhibited the synthesis and release of IL-6 and TNF-alpha in vascular endothelial cells. RNA interference targeting tryptase expression may be a new anti-inflammatory strategy for vascular diseases.</p>
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
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Down-Regulation
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Gene Expression Regulation, Enzymologic
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Cell Line
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Culture Media, Conditioned
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Transgenes
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Bodily Secretions
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RNA, Small Interfering
Limits:
Animals
Language:
En
Journal:
Journal of Zhejiang University. Science. B
Year:
2008
Type:
Article