Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 561-568, 2008.
Article
in En
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| ID: wpr-359929
Responsible library:
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ABSTRACT
<p><b>AIM</b>To show whether molecular motor dynein on a microtubule track, molecular motor myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein (MyRIP), and vesicle receptor Rab27b on an F-actin track were present during human and monkey spermiogenesis involving intramanchette transport (IMT).</p><p><b>METHODS</b>Spermiogenic cells were obtained from three men with obstructive azoospermia and normal adult cynomolgus monkey (Macaca fascicularis). Immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the proteins were carried out. Samples were analyzed by light microscope.</p><p><b>RESULTS</b>Using RT-PCR, we found that dynein, myosin Va, MyRIP and Rab27b were expressed in monkey testis. These proteins were localized to the manchette, as shown by immunofluorescence, particularly during human and monkey spermiogenesis.</p><p><b>CONCLUSION</b>We speculate that during primate spermiogenesis, those proteins that compose microtubule-based and actin-based vesicle transport systems are actually present in the manchette and might possibly be involved in intramanchette transport.</p>
Full text:
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Index:
WPRIM
Main subject:
Physiology
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Spermatids
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Spermatogenesis
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Testis
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Biological Transport
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Actins
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Myosins
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Dyneins
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Myosin Heavy Chains
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Rab GTP-Binding Proteins
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Asian Journal of Andrology
Year:
2008
Type:
Article