ADAR1 Knockout Inhibits Notch1-induced T-ALL in Mice / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 643-648, 2016.
Article
in Zh
| WPRIM
| ID: wpr-360032
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of ADAR1 on the occurrence and development of mouse T cell acute lymphoblastic leukemia (T-ALL).</p><p><b>METHODS</b>Lck-Cre; ADAR1lox/lox mice were generated through interbreeding. The lineage-cells of Lck-Cre; ADAR1lox/lox mice and the control were enriched respectively by the means of MACS, and the lin- cells were transfected with retrovirus carrying MSCV-ICN1-IRES-GFP fusion gene. Then the transfection efficiency was detected by the means of FACS, and the same number of GFP+ cells were transplanted into lethally irradiated recipient mice to observe the survival of mice in 2 recipient group after transplantation.</p><p><b>RESULTS</b>T cell-specific knockout ADAR1 mice were generated, and Notch1-induced T-ALL mouse model was established successfully. The leukemia with T-ALL characteristics occured in the mice of control group, but did not in the ADAR1 kmockout mice after transplantation.</p><p><b>CONCLUSIONS</b>ADAR1 plays a key role in the incidence and development of Notch1-induced T-ALL.</p>
Full text:
1
Index:
WPRIM
Main subject:
T-Lymphocytes
/
Adenosine Deaminase
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Mice, Knockout
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Disease Models, Animal
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Receptor, Notch1
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Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
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Genetics
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2016
Type:
Article