Immunoassay for cadmium detection and quantification / 生物医学与环境科学（英文）

ABSTRACT

<p><b>OBJECTIVE</b>To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).</p><p><b>METHODS</b>An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.</p><p><b>RESULTS</b>IC-ELISA showed an IC50 of 45.6 microg/L with a detection limit of 1.95 microg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998.</p><p><b>CONCLUSION</b>IC-ELISA can detect and quantify cadmium residue in environmental or food samples.</p>