Radon induced pulmonary lesion and expression of P53 and Bcl-2,Bax in mice / 中华放射医学与防护杂志

ABSTRACT

Objective To establish the mice model of lung injury induced by radon,and to observe the changes of pulmonary lesion at different doses and to analyze the influence of radon exposure on P53 and Bcl-2、Bax expression in lung tissue.Methods Mice were exposed to radon of 30 and 60 WLM,respectively.Apoptosis was detected by terrainal deoxynucleotidy transferse-mediated dUTP-biotin nick end labeling(TUNEL).The expressions of P53,Bcl-2 and Bax protein were observed by immunohistochemistry and Western blot.Results Compared with those in the control group,the apoptotic indexes increased significantly in the 30 and 60 WLM groups(t=18.11,-10.30,P＜0.05).The protein expression of P53 was significantly increased(t=-11.08,P＜0.05).The expression levels of P53 and Bax were remarkably inereased(t=-7.00,-2.52,P＜0.05),while the expression of Bcl-2 protein was significantly decreased in the 60 WLM group(t=4.36,P＜0.05).But Bcl-2,Bax expression was decreased in both 30 WLM and 60 WLM groups(t=2.78,4.07,P＜0.05).Conclusions Radon could induce pulmonary lesion of mice.It may be involved in the regulation of apoptosis of pulmonary lesion by the P53,Bcl-2、Bax pathway.