Role of tripterium wilfldii hook f.(T11)on pancreaticoduodenal allograft in rats / 中华器官移植杂志

ABSTRACT

Objective To study the effects of tripterium wilfordii hook f.(T11) used after transplantation on survival of pancreas graft.Methods Male Wistar rats weighing 200-300 g served as donors, while recipients were male SD rats rendered diabetes with strepotozotocin administered intravenously at a dose of 50 mg/kg body weight through tail vein.Vascularized heterotopic whole organ panereaticoduodenal transplantation was performed.The graft exocrine secretion was drained into duodenum by side-to-side anastomoses between the duodenum of donor and recipient.The insulin was drained into systemic vena by anastomoses between inferior vena cava and the infrarenal abnominal aorta of recipient and the donor aorta and portal vein.The vascular anastomoses were done by using microsurgical method.The non-fasting serum glucose and urine glucose reaction were detected at an interval of 2 days and the serum insulin Wasdetected at the 4th day post-transplantation.Results All of the recipients had normoglyeemia and negative urine glucose reaction 24 h after transplantation.The survival time of the pancreatic grafts was as follows (10.4±2.9)days in group 1,(22.0±1.5)days in group 2.Conclusion Administration of T11 after pancreatic transplantation could prolong the pancreatic graft survival.