Establishment and characterization of a multidrug resistant human mucoepidermoid carcinoma xenograft model / 实用口腔医学杂志
Journal of Practical Stomatology
; (6): 177-180, 2010.
Article
in Zh
| WPRIM
| ID: wpr-403338
Responsible library:
WPRO
ABSTRACT
Objective:To establish a multidrug resistant model of human mucoepidermoid carcinoma using nude mice. Methods:Multidrug resistant MEC/5-FU cells were inoculated intradermally into nude mice. Solid tumors were locally measurable after 10 days and 5-FU was repeated intraperitoneal injected into tumor-bearing mice. The tumor cells in nude mice (MEC/5-FU/NU) were isolated, cultured and examined. Results:The xenografts were similar to the original mucoepidermoid carcinoma from which the cell line was derived. The resistance index (RI) of the MEC/5-FU/NU cells to 5-FU was 27.82. Compared to the MEC, the expressions of ABCB1, ABCB11 and GSTA1 genes and MDR-1 protein increased in the MEC/5-FU/NU cells(P<0.05). Conclusion:The xenograft model is a good model of human multidrug resistant mucoepidermoid carcinoma, and may be useful in studying drug resistance mechanism in vivo.
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Practical Stomatology
Year:
2010
Type:
Article