The Unscheduled Expression of Cyclin B1/CDK1 Contributes to the Transition of Cell Cycle of Lymphocytes in vivo from G_0 to G_1 Phase / 华中科技大学学报(医学版)
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
;
(6): 793-795, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-404839
ABSTRACT
Objective To study the mechanism of unscheduled Cyclin B1 expression at G_1 phase which is usually at G_2/M phase.Methods Human peripheral blood lymphocytes(PBL) from healthy volunteers were firstly activated by PHA and then went into cell cycle.The cells were collected at 0,36,48 and 60 h after activation and divided into two partsone for Cyclins/DNA muhiparameter assay,and another for Post-sorting Western blot.Results After activation by PHA,Cyclin B1 and CDK1 of lymphocytes were expressed at G_1 phase.Conclusion Unscheduled Cyclin B1/CDKl probably contributes to lymphocytes in vivo into cell cycle.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
Year:
2009
Type:
Article
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