Relationship between expressions of cyclinD1, p16 and retinoblastoma gene protein and prognosis of non-small-cell lung cancer

Baosong XIE; Qiang LI; Zhongling LIU

ABSTRACT

BACKGROUND:

Disturbed regulation of cell cycle is the essential mechanism on abnormal proliferation of cell and incidence of cancer. CyclinD1 /cyclin-dependent-kinase pathway involved with p16 and retinoblastoma gene protein is an important.approach on regulation of cell cycle.

OBJECTIVE:

To understand the expressions of cyclinD1, p16 and retinohlastoma gene protein in non-small-cell lung cancer and probe into the relationship with the prognosis of cancer.

DESIGN:

Immunohistochemical assay was used to measure the expressions of relevant indexes and follow-up data were utilized for comparative analysis on survival rate and prognostic factor.

SETTING:

Department of Respiratory Disease, Fujian Provincial Hospital

PARTICIPANTS:

The experiment was performed in Department of Pathology of Fujian Provincial Hospital from January to June 2002. Tissue specimens of lung cancer were from 68 cases of non-small-cell lung cancer.Histological classification of lung cancer was based on the standards implemented by WHO in 1981 and TNM staging was on the standards revised by International Union Against Cancer (UICC) in 1997.

METHODS:

Tissue specimens of lung cancer were stained with immunohistochemical method, the positive-stained splices of lung cancer were taken as positive control and those with 1st antibody replaced by phosphate buffer were taken as negative control. The positive was identified if tumor cell nucleus or cytoplasm presented brown-yellow granules. 5 high-power visual fields were observed at randomize in each specimen and 200 tumor cells were counted and the average was obtained. According to the perceptions of positive cell, in which, positive cell count was less by 5% or no obvious positive cell appeared "-", positive cell count was in range from 5% to 20% "+", positive cell count was in range from 21% to 50% "++"and positive cell count was over 50% "(#)", the expression of cyclinD1,p16 and retinoblastoma gene protein in 68 cases of non-small-cell lung cancer were assayed.MAIN OUTCOME

MEASURES:

Expressions of cyclinD1, p16 and retinoblastoma gene protein and survival phase of patients after operation.retinoblastoma gene protein The expressive rates were 55.9%, 48.5% and 52.9% respectively. X2 was applied for analysis on the data and it was indicated that there was no remarkable correlation among their expressions.gene protein and prognosis of non-small-cell lung cancer The average survival phase of cyclinD1 positive expression was shorter than that of negative expression [(12.40, 35.40) months, (x2=5.31 ,P =0.021)]. The average survival phase of positive case of p16 positive expression was longer than that of negative one [(27.84, 15.95) months, (X2=4.38 ,P =0.036)].The average survival phase of positive expression of retinoblastoma gene protein was not more different significantly from the negative one [(18.50,of non-small-cell lung cancer with Cox proportional hazard model It was indicated that the prognosis of excessive expression of cyclinD1 was poor (P=0.057) and that of p16 only presented 1 prognostic protective factor (P=0.039).

CONCLUSION:

CyclinD1, p16 and retinoblastoma gene associated with cyclinG1-S regulation are all involved in incidence and development of non-small-cell lung cancer. The assay of expressions of cyclinD1 and p16is essentially significant for prognosis.