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Changes of glucocorticoid receptor in hepatic cytoplasm in scalded rats and its regulation by α-MSH and KPV peptide / 第三军医大学学报
Article in Zh | WPRIM | ID: wpr-410368
Responsible library: WPRO
ABSTRACT
Objective To observe the changes of glucocorticoi d receptor (GR) in hepatic cytoplasm in rats after scalding-induced pathologic al stress and its regulation. Methods The receptor binding capa city (R0) and the apparent dissociation constant (Kd) of GR in hepatic cytopla sm of normal, low-degree and heavy-degree scalded rats were measured with rad io-ligand binding assay, with [3H] dexamethasone as ligand. The changes of R0 and Kd of GR were regulated by injections of anti-rat TNFα, IL-1β a ntibodies, α-melanocyte-stimulating hormone (α-MSH), and KPV peptide( Ac- D-Lys-L-Pro-D-Val) respectively in vivo. Results The R 0 of GR in hepatic cytoplasm in rats 12 h after heavy-degree scalding [Mass action robust (205.52±30.14) fmol/mg; Scatchard (208.45±30.78) fmol/mg ]were significantly lower than that of control group [Mass action robust(307 .86±24.22) fmol/mg;Scatchard(306.71±27.96) fmol/mg](P<0.01), but no s ignificant difference was found in the R0 of GR between the control and the ra ts 12 h after low-degree scalding [Mass action robust (285.19±16.62) fmol/ mg ; Scatchard (296.64±16.06) fmol/mg]. The injection of anti-rat TNFα, IL-1β antibodies, α-MSH and KVP all prevented the decline of R0 of GR in h epatic cytoplasm in rats with severe scalding. Conclusion The injections of anti-rat TNFα, IL-1β antibodies, α-MSH or KPV can attenuate the reduction of GR in rat hepatic cytoplasm caused by severe scalding-induced pathological stress to some extent.
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2001 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2001 Type: Article