Preparation and test of type Ⅰ collagen-glycosaminoglycan (GAG) template / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 337-339, 2001.
Article
in Zh
| WPRIM
| ID: wpr-410489
Responsible library:
WPRO
ABSTRACT
Objective:
To synthesize the collagen-GAG template and to evaluate its feasibility to be used as the MSCs vehicle for meniscal tissue engineering.Methods:
The collagen-GAG template was synthesized from rat tail type Ⅰ collagen and GAG using Yannas method. Then the post-stimulated MSCs by bFGF and TGF-β1 were added in. The MSCs-enriched collagen sponges were cultured in vitro, two weeks later the histological and ultrastructure detection was performed.Results:
The histological and ultrastructure of the collagen-GAG template remained intact after 2 weeks' culture, and the MSCs in it remained viable.Conclusion:
The collagen-GAG template synthesized in this experiment is suitable for the meniscal tissue engineering reconstruction as the vehicle for MSCs seed cells.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Academic Journal of Second Military Medical University
Year:
2001
Type:
Article