Construction of Mutant N-ras/61 Gene Recombinant Vaccinia Virus / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6): 282-284, 2000.
Article
in Chinese
| WPRIM
| ID: wpr-412396
ABSTRACT
Objective:
To construct the recombinant vaccinia virus of mutant N-ras/61 gene and enhance the immunogenecity of mutant N-ras/61 protein produced by the recombinant vaccinia virus.Methods:
N-ras/61 gene was inserted into P1108 and transfected into CV-1 cell infected with vaccinia virus by Cell FECTIN. PCR and Western blot were used to identify the recombinants.Results:
We get recombinant vaccinia virus rV-N-ras/61 by PCR and tk- selecting. The expression of N-ras gene was detected by Western blot.Conclusion:
This study is a test for studing effective vaccine of mutant N-ras/61 gene. The efficacy in vivo of the N-ras/61 vaccine in immunotherapy is under investgation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
2000
Type:
Article
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