A modified isolation method for mouse intestinal intraepithelial lymphocytes / 肠外与肠内营养
Parenteral & Enteral Nutrition
; (6): 106-108, 2010.
Article
in Zh
| WPRIM
| ID: wpr-415291
Responsible library:
WPRO
ABSTRACT
Objective:
To explore and modify the isolation method for mouse intestinal intraepithelial lymphocytes.Methods:
Epithelium mucosae of mouse small intestine was incubated in iced bath and shaked in PBS containing DTT. The cell suspension was obtained after filtration with 80 and 400- screen mesh trap valve in turn. The yield, viability and purity of intestinal intraepithelial lymphocytes were observed to estimate the feasibility.Results:
About (5.6±0.7)×10~6 intestinal intraepithelial lymphocytes were obtained from every 20cm samll intestine. The viabilty of intestinal intraepithelial lymphocytes was (90.46±5.71)% and the purity was (92.21±5.20)%.Conclusion:
Compared with other reported isolation methods, the modifled method is convenient and esay to handling.The yield, viability and purity are high enough to be used for intestinal intraepithelial lymphocytes studies.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Parenteral & Enteral Nutrition
Year:
2010
Type:
Article