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Expression of CD28-T-cell subtypes in peripheral blood and synovial fluid of patients with rheumatoid arthritis / 中华风湿病学杂志
Chinese Journal of Rheumatology ; (12): 611-614, 2011.
Article in Chinese | WPRIM | ID: wpr-420631
ABSTRACT
ObjectiveTo investigate the expression and significance of CD28- cells, CD4+ and CD8+T lymphocytes in the peripheral blood and synovial fluid in patients with rheumatoid arthritis ( RA ). Methods The expression of CD28, CD4+, CD8+ T lymphocytes and inducible co-stimulator(ICOS) in the peripheral blood and synovial fluid in 45 patients with RA were detected by three-color flow cytometry. Independent sample's t test was used for statistical analysis between the two groups. ResultsSynovial fluid CD4+CD28+ICOS+, CD4+CD28- ICOS+ , CD8 + CD28 + , CD8 + CD28 + 1COS+ T lymphocytes were significantly increased than the peripheral blood in RA patients[(36±19)% vs (15±8)%, t=-4.234, P<0.01; (2.1±2.2)% vs (0.6±1.4)%, t=-3.143, P<0.01; (62±15)% vs (47±18)%, t=-2.885, P<0.01; (9±9)% vs (3±3)%,t=-2.131, P<0.05], Synovial fluid CD8+CD28-T lymphoc-ytes were significantly reduced than the peripheral blood[(38±15)% vs (54±18)%, t=2.975, P<0.01], Synovial fluid CD8+ CD28-ICOS+, CD4+CD28+and CD4+ CD28- T lymphocytes had no significant difference than the peripheral blood (P>0.05). Compared with peripheral blood in the same patients with RA, CD4+CD28+ ICOS+, CD8+ CD28+ T lymphocyteswere significantly increased[(38±18)% vs (16±10)%, t=-4.065, P<0.01 ; (61±16)% vs (41±21)%, t=-4.065,P<0.01], CD8+CD28-T was significantly reduced[(39±16)% vs (59±21)%, t=2.949, P<0.01]. The level of CD4+ CD28-, CD8+ CD28-, CD28-ICOS+ T lymphocytes in the active and remission patients with RA was not significantly different (P>0.05). ConclusionSynovial fluid CD28T lymphocyte subsets disturbance and the abnormal expression of ICOS in patients with RA may play important roles in the mechanism of joint damage.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Rheumatology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Rheumatology Year: 2011 Type: Article