The influence of down-regulation of Tspan 8 by shRNA on metastasis and invasion of hepatocellular carcinomas / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 126-129, 2012.
Article
in Zh
| WPRIM
| ID: wpr-424889
Responsible library:
WPRO
ABSTRACT
Objective To study the effect of Tspan 8 on metastasis and invasion of human hepatocellular carcinomas(HCC).Methods RT-PCR and western blot were used to detect the expressions of Tspan 8 in HCC cell lines,HCC and matched nontumorous tissues.The expression of Tspan 8 was then down-regulated by LV/GFP/Tspan 8 in HCC cells.The expressions of Tspan 8 mRNA and protein were determined by RT-PCR and Western blot assay,respectively.The proliferation was examined by MTT,the expression of AMDM12 was assessed by Western blot,and the invasion ability of HCC cells was evaluated by transwells.Results A high level of Tspan 8 was found in high metastatic potential HCC cells,and the expression of Tspan 8 in HCC tissues was much higher than that in the matched nontumorous tissues. Down-regulation of Tspan 8 had no influence on the proliferation of HCC cells (P>0.05),while it inhibited the expression of ADAM12 and the invasive ability of HCC cells (P<0.01,P<0.01 respectively).Conclusion Tspan 8 played an important role in invasion and metastasis of human hepatocellular carcinomas and down-regulation by LV/GFP/Tspan 8 inhibited the invasiveness of human hepatocellular carcinoma cells.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Hepatobiliary Surgery
Year:
2012
Type:
Article