Evaluation of a side population of canine lymphoma cells using Hoechst 33342 dye
Journal of Veterinary Science
; : 481-486, 2013.
Article
in En
| WPRIM
| ID: wpr-43058
Responsible library:
WPRO
ABSTRACT
Cancer stem cell (CSC) research has increased exponentially to gain further insight into the mechanisms underlying both carcinogenesis and chemotherapy resistance. The present study was performed to explore the potential value of a side population (SP) assay for identifying and characterizing putative CSCs among canine lymphoma cells. Canine lymphoma cells from cell lines and clinical samples were subjected to the SP assay consisting of Hoechst 33342 staining and subsequent flow cytometric analysis. The SP assay revealed various amounts of a SP fraction among the canine lymphoma cells. The percentages of SP were not affected by inhibitors of membrane transporters, verapamil hydrochloride, or fumitremorgin C. Most of the canine lymphoma cells expressed high levels of Bmi-1 and membrane transporter proteins such as ABCG2 and phosphorylated (p)-glycoprotein. This investigation lays the groundwork for further studies of the biological behaviors and molecular characteristics of CSCs in cases of canine lymphoma.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Neoplastic Stem Cells
/
Benzimidazoles
/
Gene Expression Regulation, Developmental
/
Cell Line, Tumor
/
Dog Diseases
/
Side-Population Cells
/
Flow Cytometry
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Fluorescent Dyes
/
Lymphoma
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2013
Type:
Article