Astaxanthin induces migration in human skin keratinocytes via Rac1 activation and RhoA inhibition
Nutrition Research and Practice
; : 275-280, 2017.
Article
in En
| WPRIM
| ID: wpr-44076
Responsible library:
WPRO
ABSTRACT
BACKGROUND/OBJECTIVES:
Re-epithelialization has an important role in skin wound healing. Astaxanthin (ASX), a carotenoid found in crustaceans including shrimp, crab, and salmon, has been widely used for skin protection. Therefore, we investigated the effects of ASX on proliferation and migration of human skin keratinocyte cells and explored the mechanism associated with that migration. MATERIAL/METHOD:
HaCaT keratinocyte cells were exposed to 0.25-1 µg/mL of ASX. Proliferation of keratinocytes was analyzed by using MTT assays and flow cytometry. Keratinocyte migration was determined by using a scratch wound-healing assay. A mechanism for regulation of migration was explored via immunocytochemistry and western blot analysis.RESULTS:
Our results suggest that ASX produces no significant toxicity in human keratinocyte cells. Cell-cycle analysis on ASX-treated keratinocytes demonstrated a significant increase in keratinocyte cell proliferation at the S phase. In addition, ASX increased keratinocyte motility across the wound space in a time-dependent manner. The mechanism by which ASX increased keratinocyte migration was associated with induction of filopodia and formation of lamellipodia, as well as with increased Cdc42 and Rac1 activation and decreased RhoA activation.CONCLUSIONS:
ASX stimulates the migration of keratinocytes through Cdc42, Rac1 activation and RhoA inhibition. ASX has a positive role in the re-epithelialization of wounds. Our results may encourage further in vivo and clinical study into the development of ASX as a potential agent for wound repair.Key words
Full text:
1
Index:
WPRIM
Main subject:
Pseudopodia
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Salmon
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Skin
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Wound Healing
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Wounds and Injuries
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Immunohistochemistry
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Carotenoids
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Keratinocytes
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Cell Movement
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Blotting, Western
Limits:
Humans
Language:
En
Journal:
Nutrition Research and Practice
Year:
2017
Type:
Article