ASPS induces G_2/M arrest of H446 cells by activation of ERK signal pathway / 基础医学与临床
Basic & Clinical Medicine
; (12): 59-62, 2010.
Article
in Zh
| WPRIM
| ID: wpr-441317
Responsible library:
WPRO
ABSTRACT
Objective To investigate ASPS induced G_2/M arrest in lung cancer cell line H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis was used to inspect the proteins of ERK, p-ERK. Results Compared with control group, G_2/M phase cells increased with concentration significantly, G_0/G_1 phase cells were not different, G_2/M phase cells and G_0/G_1 phase cells were not different when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, protein of p-ERK was significantly increased, expression of ERK was no different. Conclusion ASPS may induce G_2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.
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WPRIM
Language:
Zh
Journal:
Basic & Clinical Medicine
Year:
2010
Type:
Article