Influence of low level, long-term arsenite exposure on HaCat cell apoptosis and the expression of Bcl-2 and Bax proteins / 中华地方病学杂志
Chinese Journal of Endemiology
; (12): 21-23, 2014.
Article
in Zh
| WPRIM
| ID: wpr-444143
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ABSTRACT
Objective To investigate the influence of low-dose,long-term arsenite exposure on keratinocyte (HaCat) cell apoptosis and the expression of Bcl-2 and Bax proteins.Methods HaCat cells were exposed to different concentrations of NaAsO2[0(control group),0.05,0.10 μmol/L] for 15 weeks.A total of 10 000 cells in each group were measured to detect the level of apoptosis by flow cytometry.The protein expressions of Bcl-2 and Bax were determined by Western blotting method.Results The cell apoptosis rate was significantly different between groups (F =17.19,P < 0.05).Compared with that of the control [(1.42 ± 0.13)%],the apoptosis rates of the 0.05 and 0.10 μmol/L groups [(1.23 ± 0.08)% and (1.04 ± 0.06)%] decreased significantly (all P < 0.05); the 0.10 μmol/L group was significantly lower than that of the 0.05 μmol/L group(P < 0.05).The protein expressions of Bcl-2 and Bax were significantly different between groups (F=107.38,346.45,all P< 0.05).The protein expression of Bcl-2 of the 0.05 and 0.10 μmol/L groups were (143.89 ± 7.74)% and (199.96 ± 12.18)%,respectively,which were significantly higher than that of the control group[(100.00 ± 1.45)%,all P < 0.05] ; the 0.10 μmol/L group was significantly higher than that of the 0.05 μmol/L group (P < 0.05).The protein expression of Bax of the 0.05 and 0.10 μmol/L groups were (70.78 ± 1.53)% and (54.80 ± 1.34)%,respectively,which were markedly lower than that of the control group[(100.00 ± 3.09)%,all P < 0.05]; the 0.10 μmoL/L group was significantly lower than that of the 0.05 μmol/L group(P < 0.05).Conclusion Decreased level of apoptosis induced by low level and long-term arsenic exposure may be associated with increased expression of Bcl-2 protein and decreased expression of Bax protein.
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Language:
Zh
Journal:
Chinese Journal of Endemiology
Year:
2014
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Article