Involvement of the Ca2+ signaling pathway in osteoprotegerin inhibition of osteoclast differentiation and maturation
Journal of Veterinary Science
; : 151-156, 2015.
Article
in En
| WPRIM
| ID: wpr-44467
Responsible library:
WPRO
ABSTRACT
The purpose of this study was to determine whether the Ca2+ signaling pathway is involved in the ability of osteoprotegerin (OPG) to inhibit osteoclast differentiation and maturation. RAW264.7 cells were incubated with macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-kappaB ligand (RANKL) to stimulate osteoclastogenesis and then treated with different concentrations of OPG, an inhibitor of osteoclast differentiation. The intracellular Ca2+ concentration [Ca2+]i and phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in the different treatment groups were measured by flow cytometry and Western blotting, respectively. The results confirmed that M-CSF + RANKL significantly increased [Ca2+]i and CaMKII phosphorylation in osteoclasts (p < 0.01), and that these effects were subsequently decreased by OPG treatment. Exposure to specific inhibitors of the Ca2+ signaling pathway revealed that these changes varied between the different OPG treatment groups. Findings from the present study indicated that the Ca2+ signaling pathway is involved in both the regulation of osteoclastogenesis as well as inhibition of osteoclast differentiation and activation by OPG.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Osteoclasts
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Cell Differentiation
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Cell Line
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Cell Survival
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Gene Expression Regulation
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Calcium
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Macrophage Colony-Stimulating Factor
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Calcium Signaling
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RANK Ligand
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Osteoprotegerin
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2015
Type:
Article