Effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1 / 中西医结合学报
Journal of Integrative Medicine
; (12): 388-91, 2006.
Article
in Zh
| WPRIM
| ID: wpr-449664
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To observe the effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1 in vitro, and to explore its mechanism.METHODS:
The human renal cell carcinoma cell line GRC-1 was treated with matrine of different concentrations for 24, 48, 72 and 96 h respectively. The MTT assay was used to evaluate the cytotoxic effects of matrine on GRC-1 cells. The transmission electron microscope and flow cytometry were utilized to observe and detect the apoptosis of GRC-1 cells induced by matrine. The expression levels of Bcl-2 and Bax proteins were evaluated by streptavidin-biotin-peroxidase method.RESULTS:
The matrine of different concentrations all have cytotoxic effects on GRC-1 cells, with obvious dose- and time-dependent effects. The apoptosis induced by matrine was confirmed in GRC-1 cells. With intervention of matrine (1.5 g/L) for 12 h, the expression level of Bcl-2 in GRC-1 cells was decreased while the expression level of Bax was increased as compared with those in the untreated group.CONCLUSION:
The proliferation-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1 may be related to down-regulating the ratio of Bcl-2/Bax protein expression and promoting the apoptosis.
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Integrative Medicine
Year:
2006
Type:
Article