The Change of Bone Metabolism in Ovariectomized Rats : Analyses of MicroCT Scan and Biochemical Markers of Bone Turnover
Journal of Korean Neurosurgical Society
; : 323-327, 2012.
Article
in En
| WPRIM
| ID: wpr-45153
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
The purpose of this study was to verify the appropriateness of ovariectomized rats as the osteoporosis animal model.METHODS:
Twelve female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy [the ovariectomy (OVX) group]. Eight weeks after operations, serum biochemical markers of bone turnover were analyzed; osteocalcin and alkaline phosphatase, which are sensitive biochemical markers of bone formation, and C-terminal telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption. Bone histomorphometric parameters and microarchitectural properties of 4th lumbar vertebrae were determined by micro-computed tomographic (CT) scan.RESULTS:
The OVX group showed on average 75.4% higher osteocalcin and 72.5% higher CTX levels than the sham group, indicating increased bone turnover. Micro-CT analysis showed significantly lower bone mineral density (BMD) (p=0.005) and cortical BMD (p=0.021) in the OVX group. Furthermore, the OVX group was found to have a significantly lower trabecular bone volume fraction (p=0.002).CONCLUSION:
Our results showed that bone turnover was significantly increased and bone mass was significantly decreased 8 weeks after ovariectomy in rats. Thus, we propose that the ovariectomized rat model be considered a reproducible and reliable model of osteoporosis.Key words
Full text:
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Index:
WPRIM
Main subject:
Osteogenesis
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Osteoporosis
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Bone Resorption
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Ovariectomy
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Biomarkers
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Bone Density
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Osteocalcin
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Salicylamides
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Rats, Sprague-Dawley
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Collagen Type I
Limits:
Animals
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Female
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Humans
Language:
En
Journal:
Journal of Korean Neurosurgical Society
Year:
2012
Type:
Article