The clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 2216-2217, 2014.
Article
in Zh
| WPRIM
| ID: wpr-456067
Responsible library:
WPRO
ABSTRACT
Objective To investigate the clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers.Methods 653 hepatitis B patients in our hospital from 2013 April to 2013 December were selected.First ELISA method using HBV-M model for qualitative detection of the blood samples,the detection order:Hepatitis b virus surface antigen (HBsAg),Hepatitis B surface Antibody(HbsAb),Hepatitis B e Antigen(HbeAg),hepatitis Be antibody(HbeAb),hepatitis B core antibody(HbcAb);Then the FQ-PCR method for the quantitative detection of HBV-DNA,different HBV-m model test results were compared..Results Big 3 this world[1(+)、3 (+)、5 (+)model]and[1 (+)、3 (+)model ]of the HBV-DNA positive rate was 97.97%,94.74% was significantly higher than that in other mode(P<0.05).Big 3 this world[1(+)、3(+)、5(+)model]HBV-DNA expression level is the highest(5.59 ×106 ±2.42 ×105 )copies/mL was significantly higher than that in other mode(P<0.05).Conclusion Combined with qualitative and quantitative detection of HBV-DNA HBV-M,which is of great value to clinical early diagnosis and therapy.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
/
Qualitative_research
/
Screening_studies
Language:
Zh
Journal:
International Journal of Laboratory Medicine
Year:
2014
Type:
Article