Construction and activity characterization of E-cadherin and N-cadherin promoters / 军事医学
Military Medical Sciences
;
(12): 860-862, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-458750
ABSTRACT
Objective To clone E-cadherin and N-cadherin promoters and insert them into a luciferase reporter gene vector, and to characterize the promoter activity of E-cadherin and N-cadherin.Methods E-cadherin and N-cadherin pro-moter were cloned into pGL 4-basic.The resulting plasmids were determined by DNA sequencing .The promoter activity was analyzed in breast cancer cell line ZR 75-1 and hepatocarcinoma cell line HepG 2.Results DNA sequencing showed that the sequences of the cloned promoter regions were correct .Analysis of the reporter gene activity indicated that the E-cad-herin and N-cadherin promoters had the highest transcriptional activity in ZR 75-1 and HepG2 cells.Conclusion The E-cadherin and N-cadherin promoter genes are cloned successfully , contributing much to screening transcription factors that regulate E-cadherin and N-cadherin expression .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Military Medical Sciences
Year:
2014
Type:
Article
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