An efficient method which can be used to transfect Tibetan minipig embryonic fibroblasts / 中国比较医学杂志
Chinese Journal of Comparative Medicine
; (6): 64-66, 2015.
Article
in Zh
| WPRIM
| ID: wpr-464557
Responsible library:
WPRO
ABSTRACT
Objective To transfect EGFP gene to porcine embryonic fibroblasts ( PEFs) of Tibetan miniature pigs by Lonza Nucleofector II machine and compare the tansfection efficiency between this method and the lipofection method. Method A plasmid carrying green fluorescent protein ( GFP) was transfected into PEFs of Tibetan miniature pigs via the Lonza Nucleofector II machine ( program U020) and by Lipofectamine 2000.Results 5 hours after nucleofection, green fluorescence was observed, indicating 80%transfecting efficiency in the nucleofection group, which is significantly higher than the lipofection group. Conclusion Nucleofector II machine can efficiently transfect PEFs, provides a reliable method for efficiently generate transgenic Tibetan minipigs.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Comparative Medicine
Year:
2015
Type:
Article