Sequence analysis of a novel HLA-A?01 ∶130 allele and modeling of three-dimensional structure of HLA molecule / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 514-516, 2015.
Article
in Zh
| WPRIM
| ID: wpr-464972
Responsible library:
WPRO
ABSTRACT
Objective:
To confirm the novel allele HLA-A*01∶130 and analyzed the nucleotide sequence of the abnormal reaction pattern.Methods:
The HLA typing of sample DNA was performed by PCR-SBT.The ambiguous novel HLA allele was confirmed with single stranded SBT method,then DNA sequencing was performed to identify the difference between the novel allele and HLA-A?0166 allele.Finally, it was modeled by Swiss-Model to three-dimensional structure of HLA Molecule.Results:
The novel allele was not the same with all known HLA-A allele sequence.After analysis,there was one nucleotide differed from the A?0166 at position 368 where A→G( codon 99 TAT→TGT) resulting in a coding change,99 Tyr was changed to Cys.The amino acid substitution at residue 99 the HLA polypeptide was located in a beta-sheet of antigenic peptide-binding region.Conclusion:
The allele is a novel allele that has now been officially named as HLA-A*01∶130 by the World Health Organization( WHO) HLA Nomenclature Committee.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
2015
Type:
Article