In-vitro study of glycogen synthase kinase-3β on regulation of migration, invasion and epithelial-mesenchymal transition in pancreatic cancer cells / 中华普通外科杂志
Chinese Journal of General Surgery
;
(12): 853-856, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-468775
ABSTRACT
Objective To explore the role of GSK-3β in migration,invasion,metastasis and epithelial-mesenchymal transition of pancreatic cancer in vitro.Methods Lentiviral interference was used to inhibit the expression of GSK-3β in pancreatic cancer cells.Western blotting was used to detect expression of GSK-3β phosphorylation molecules and epithelial-mesenchymal transition and invasion related molecule.Scratch test and transwell test were adopted to detect the effect of GSK-3β inhibition on cell migration and invasion in pancreatic cancer cells.Luciferase reporter gene test was used to detect the effect of GSK-3β inhibition on NF-κB binding activity.Results Inhibition of GSK-3β expression significantly restrain migration,invasion and epithelial-mesenchymal transformation.Migration inhibitory rate was 59.1% ±6.4% in ASPC-1 group and 55.9% ± 7.3% in PANC-1 group.GSK-3β inhibition affected the activity of NF-κB,the binding activity decreased to 24.8% ± 3.1% and 31.5% ±5.4%,respectively (all P < 0.05).Conclusions GSK-3β participates in the regulation of migration,invasion,and epithelialmesenchymal transition of pancreatic cancer.GSK-3β inhibition could surpress proliferation,invasion and epithelial-mesenchymal transformation,and NF-κB might be the key molecule in the regulation of pancreatic malignant behavior.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of General Surgery
Year:
2014
Type:
Article
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