Study on Drug Pair of Cassia Twig and White Peony Root by HPLC/MS Analysis Based on Theory of“Xin-Gan Hua-Yang and Suan-Gan Hua-Yin” / 世界科学技术-中医药现代化

ABSTRACT

This article was aimed to study the different clinical characteristics using drug pair of Cassia twig and white peony root with the contents ratio of 11 and 12. Based on the different clinical treatment of drug pair of Cas-sia twig and white peony root, different compositional ingredients in ratio of 11 and 12 were illuminated by HPLC/MS method. The drug pair of Cassia twig and white peony roots in ratio of 11 and 12 and single herbs were ex-tracted for HPLC/MS analysis. A protocol was followed, including acetonitrile - 0.1% acetic acid with gradient elution, positive mode, 350℃ capillary temperature and 300℃ vaporization temperature. The results showed that Procyanidol B2 and 2-Hydroxy cinnamal dehyde can be extracted from single Cassia twig, but 2-Hydroxy cinna-mal dehyde cannot be detected in drug pair. It showed the contents of Procyanidol B2 in 11 ratio was more than 12 ratio. Simultaneously, Palbinone, paeoniflorin sulfonate, 1,2,3,6-Tetra-O-galloyl-β-D-glucose, Paeoniflorin, Pae-oniflorin isomers, Benzoylpaeo-niflorin, and Benzoyl Paeoniflorin isomers can also be dissolved in white peony root. In addition, the contents of 1,2,3,6-Tetra-O-galloyl-β-D-glucose, Paeoniflorin, Benzoylpaeo-niflorin, and Benzoyl Paeoniflorin isomers in 11 were more than 12. The contents of Palbinone, paeoniflorin sulfonate and Paeoniflorin isomers in 12 were more than 11. It was concluded that Procyanidol B2, 1,2,3,6-Tetra-O-galloyl-β-D-glucose, Paeoniflorin, Benzoylpaeo-niflorin and Benzoyl Paeoniflorin isomers in 11 were more than 12. The contents of Pal-binone, Paeoniflorin sulfonate and Paeoniflorin isomers in 12 were more than 11. It provided a scientific basis for traditional Chinese medicine treatment using rational drug pair.