Effects of Selective Cyclooxygenase-2 Inhibitor NS-398 Pretreatment on the Rat Spinal Cord after Contusion Injury
Korean Journal of Pathology
;
: 255-262, 2006.
Article
in English
| WPRIM
| ID: wpr-47610
ABSTRACT
BACKGROUND:
Secondary spinal cord injury (SCI) that follows an initial mechanical insult can exacerbate the overall damage, limit the restorative processes and eventually lead to an in- creased neurological deficit. We hypothesized that selective inhibition of cyclooxygenase-2 (COX-2) may decrease the delayed cell death, and so this will contribute to decreased level of the secondary injury.METHODS:
The dorsal surface of the cord at the T9 level was subjected to weight drop impact using a 10 g rod. To block COX-2 activation, a selective COX-2 inhibitor (NS-398) was administered (5 mg/kg, i.p.) 15 min prior to SCI. The COX-1, COX-2, Caspase-3 and PGE2 expressions were measured by real time quantitative RT-PCR and fluorescence immunostaining.RESULTS:
Many activated caspase-3 positive cells were observed at 6 h and they increased until 72 h after SCI. The expression of COX-2 peaked at 6 h after SCI, while the COX-1 expression was unaffected. The principal cells that showed a COX-2 expression were the neurons and microglia. Pretreatment with NS-398 caused a significant decrease in the expression of prostaglandin E2 and activated caspase-3 positive cells after SCI.CONCLUSION:
These data suggest that COX-2 is one of the main factors related with the pathologic deficits from secondary SCI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spinal Cord
/
Spinal Cord Injuries
/
Dinoprostone
/
Cell Death
/
Microglia
/
Contusions
/
Cyclooxygenase 2
/
Cyclooxygenase 2 Inhibitors
/
Caspase 3
/
Fluorescence
Limits:
Animals
Language:
English
Journal:
Korean Journal of Pathology
Year:
2006
Type:
Article
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