Expression and regulation of Bim protein in chronic myelogenous leukemia / 中国药科大学学报

ABSTRACT

Aim: To investigate the expression and the regulation of Bim protein in leukemic cell of patient with chronic myelognous leukemia(CML). Methods: The primary leukemic cells from the peripheral blood or bone marrow of patients with CML were isolated and cultured. The expression of Bim in the present or absence of ima-tinib(BCR/ABL inhibitor) was detected by the Western blotting, immunohistochemical analysis and real-time quantitative PCR. Results: Bim was consecutively expressed in normal bone marrow, but significantly decreased in the bone marrow of patients with CML( P < 0. 05). Imatinib significantly enhanced Bim mRNA transcription and expression in the manner of time- and dose-dependence. Moreover, there was reverse correlation between Bim protein level and the percent of leukemic cell in bone marrow( r =0. 849 1, P <0. 05). Conclusion: Bim is the Bcl-2 family pro-apoptitic factor. The decreased expression of Bim could be vital in the CML, Bim protein up-expression following the suppression of BCR/ABL might be of importance in investigation of the Bim-induced ap-optosis for potential clinical therapy.