Simultaneous Determination of Three Active Ingredients inTongjingling Granules by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish a simultaneous determination method for Salvianolic acid B, Paeoniflorin and Hydroxysafflor yellow A inTongjingling Granules; To provide a reliable method for effective quality control. Methods The determination was performed on a Welchrom C18 column with acetonitrile (A) - 0.1% phosphoric acid (B) as the mobile phase in gradient elution mode at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm. Results Salvianolic acid B presented in a good linear relation in the range of 0.197–1.316μg, and the average recovery rate was 98.72% with RSD 1.49%; Paeoniflorin presented in a good linear relation in the range of 0.158–1.051μg, and the average recovery rate was 99.01% with RSD 0.92%; Hydroxysafflor yellow A presented in a good linear relation in the concentration range of 0.053–0.353μg, and the average recovery rate was 98.34% with RSD of 1.60%.Conclusion This method is simple, sensitive, accurate and with good reproducibility, which can be used for quality control ofTongjingling Granules.