Simultaneous Determination of Chlorogenic Acid and Mangiferin in Yinhua Mangguo Tablets by Wave-length Switching HPLC / 中国药师

ABSTRACT

Objective:To establish a wavelength switching HPLC method for the simultaneous determination of chlorogenic acid and mangiferin in Yinhua Mangguo tablets. Methods:An Agilent ZORBAX Eclipse XDB-C18 (150 mm × 4. 6 mm, 5 μm) column was used. The mobile phase was methanol-water containing 0. 2% phosphoric acid with gradient elution at the flow rate of 1. 0 ml· min-1;the detection wavelength was 327 nm(0-8 min) for chlorogenic acid and 258 nm(8-20 min) for mangiferin. Results:Chlorogenic acid had good linearity within the range of 0. 140-2. 516 μg, and the average recovery was 99. 4% with RSD of 1. 2% (n=9). Mangiferin had good linearity within the range of 0. 042-0. 761 μg, and the average recovery was 99. 9% with RSD of 1. 4% (n=9). Conclu-sion:The method is simple, accurate and reproducible. It can be used to determine chlorogenic acid and mangiferin in Yinhua Mang-guo tablets with the same chromatogram conditions.