Establishment of a specific gene diagnosis method for Yersinia pestis by quantitative real-time PCR / 中华地方病学杂志
Chinese Journal of Endemiology
; (12): 119-122, 2016.
Article
in Zh
| WPRIM
| ID: wpr-489858
Responsible library:
WPRO
ABSTRACT
Objective Using quantitative real-time PCR to establish a rapid specific genetic diagnostic technique for Yersinia pestis.Methods ①Four sets of specific probes and primers were designed,which targeted to chromosome genes of YPO0392,YPO1094,YPO2087 and YPO2090,respectively.②The probes and primers were tested for stability and specificity with 40 strains of Yersinia pestis and 47 strains of non-Yersinia pestis of different sources in Yunnan.③Eight positive DNA in Yulong,Yunnan,were tested with the screened probes and primers.Results ①Two sets probes and primers were selected,they were targeting YPO0392 and YPO1094,respectively.②The results were all positive of the eight positive DNA samples tested.Conclusion Two sets of primers and probes are selected for rapid specific diagnosis of Yersinia pestis.
Full text:
1
Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Endemiology
Year:
2016
Type:
Article