Comparison of volatile constituents in two types of mugwort leaves (produced in Qichun and Nanyang) using the headspace GC-MS / 针灸推拿医学（英文版）

ABSTRACT

Objective:To compare the volatile constituents in mugwort leaves produced in Qichun, Hubei Province and Nanyang, Henan Province. Methods:The volatile constituents were extracted using headspace heating and analyzed using gas chromatography-mass spectrometry (GC-MS). Then a qualitative analysis was made according to the standard database provided by the National Institute of Standards and Technology (NIST) and the relative contents of each constituent were calculated using the peak area normalization method. Results:A total of 59 compounds were identified from the mugwort leaves from Qichun and 51 compounds were identified from the mugwort leaves from Nanyang. These mainly include monoterpenoids, sesquiterpenoids, CxHyOz and other compounds involving the aldehyde, ketone, alkane and benzene. The mugwort leaves from Qichun and Nanyang share 32 common volatile constituents. The chromatographic peak area of identified compounds accounting for 96.38% of GC-MS total chromatographic peak areain Qichun mugwort leaves, versus 95.54% of that in Nanyang mugwort leaves. Conclusion:The headspace heating extraction combined with GC-MS technology can evidently display similarities and differences of volatile constituents in mugwort leaves produced in different areas and thus provide scientific basis for the quality and screening of mugwort leaves.