Protective Effect of Diclipterachinensis Polysaccharide P2 B on Carbon Tetrachloride-induced Injury of Liv-er Cell Line L-02 / 中国药师
China Pharmacist
; (12): 675-677, 2016.
Article
in Zh
| WPRIM
| ID: wpr-490922
Responsible library:
WPRO
ABSTRACT
Objective:To evaluate the protective effect of Diclipterachinensis polysaccharide P2B on liver cell line L-02 injury in-duced by carbon tetrachloride ( CCl4 ) . Methods:The human liver L-02 cells were cultured, and the injury model was built by CCl4 . The L-02 cells were divided into the normal control group, the CCl4-damaged group, and the P2B sample groups (0. 125, 0. 250 and 0. 500 mg· ml-1 ). The contents of alanine aminotransferase ( ALT), aspartate aminotransferase ( AST), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined by MTT assay. Results:Compared with the CCl4-damaged group, P2B could improve the activity of L-02 cells, and the activity of AST and ALT in the supernatant was significantly reduced, and the content of SOD in the cells was increased and that of MDA was decreased. Conclusion:P2B can significantly prevent L-02 cells from the damage induced by CCl4 in a dose-dependent manner, and the mechanism may be related with the anti-oxidative activity of P2B.
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WPRIM
Language:
Zh
Journal:
China Pharmacist
Year:
2016
Type:
Article