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Establishment of Congenital Heart Disease Transposition of Great Arteries in Experimental Mice Model / 中国循环杂志
Chinese Circulation Journal ; (12): 499-501, 2016.
Article in Zh | WPRIM | ID: wpr-492587
Responsible library: WPRO
ABSTRACT
Objective: To establish a mice model of congenital heart disease transposition of great arteries in order to provide a research reference for the occurrence and development of transposition of great arteries. Methods: A total of 20 pregnant ICR mice at 8-10 weeks of age were divided into 2 groups: Control group, the mice received a single dose of DMSO 70 mg/kg at 8.5 days of gestation,n=5 and Experiment group, the mice received a single dose of all-trans retinoic acid 70 mg/kg at 8.5 days of gestation,n=15. All animals were treated for 18 days and then the embryos were taken to observe cardiac morphology under stereomicroscope. Results: Compared with Control group, Experiment group had obviously increased occurrence rates of premature delivery, abortion and embryo absorption, and 61.2% phenotype for transposition of great arteries; meanwhile, combining with non-heart defect phenotypeas exophthalmos and spinal malformation. Conclusion: All-trans retinoic acid may induce transposition of great arteries in mice embryos, which is a feasible animal model in experimental research.
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Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Circulation Journal Year: 2016 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Circulation Journal Year: 2016 Type: Article