Metabolic analysis of immune suppression mice based on NMR / 中国药理学通报

ABSTRACT

Aim Toanalyzethecharacteristicsofser-um metabolites in immune suppression mice by using 1HNMR-basedmetabonomics.Methods Twentymale Kunming(KM)mice were randomly divided into two groupsnormal group and cyclophosphamide (Cy ) model group.Cy model group was given Cy to induce immune suppression.NMR technology was used to find out the variability of serum metabolites by the method ofOPLS-DA.Results Comparedwithnormalgroup, Cy model group showed a significant decrease in the serum levels of glutamine,glycolprotein,unsaturated lipid,VLDL,LDL,acetone and showed a significant increase in the serum levels of leucine,alanine,tyro-sine, histidine, lactate, glycine, creatine, me-theionine,valine,β-hydrocxy butyrate and carnitine. Conclusions Immunesuppressionmiceareimbal-anced in metabolic network.These findings indicate that lipid metabolism-related metabolites are de-creased,however,β-oxidation of fatty acid,proteoly-sis and glycolysis level are increased.