Effects of Quercetin on Human Lung Cancer NCI-H1395 Cell Apoptosis / 中国药房
China Pharmacy
; (12): 4786-4788, 2015.
Article
in Zh
| WPRIM
| ID: wpr-501206
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To investigate the effects of quercetin on human lung cancer NCI-H1395 cell apoptosis.METHODS:
CCK-8 was used to detect the effects of 0-200 μmol/L quercetin on human lung cancer NCI-H1395 cell proliferation after treated for 12,24 and 48 h. Hochest33258 staining and flow cytometry were used to detect the effects of 0,20,50,100 μmol/L quercetin on NCI-H1395 cell apoptosis after treated for 24 h. The effects of 100 μmol/L quercetin on NCI-H1395 cell apoptosis was investi-gated after treated with Caspase-8,Caspase-9,Caspase-3 inhibitor.RESULTS:
Quercetin could inhibit NCI-H1395 cell prolifera-tion in dose and time-dependent manner. 20,50,100 μmol/L quercetin could induce the apoptosis of NCI-H1395 cell,and apoptot-ic rates were (18.6 ± 4.1)%,(39.1 ± 4.5)% and (58.2 ± 3.5)%. Caspase-8 and Caspase-3 activation inhibition could obviously weaken the inhibitory effects of quercetin on cell(P<0.05).CONCLUSIONS:
Quercetin can inhibit NCI-H1395 cell proliferation and induce cell apoptosis,which is related to the external way of cell apoptosis through activating Caspase-8 and Caspase-3.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2015
Type:
Article