Simultaneous Contents Determination of Chloramphenicol and Diphenhydramine Hydrochloride in Chloro-benzene Alcohol Solution by HPLC / 中国药房
China Pharmacy
;
(12): 3847-3849, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-503519
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous contents determination of chloramphenicol and diphenhydr-amine hydrochloride in Chlorobenzene alcohol solution.METHODS:
HPLC was performed on the column of Diamonsil C with mo-bile phase of phosphate buffer-acetonitrile(66.5∶33.5,V/V)containing 0.01 mol/L sodium heptane,column temperature was 35 ℃, flow rate was 1 ml/min,detection wavelength was 258 nm for diphenhydramine hydrochloride and 277 nm for chloramphenicol, the injection volume was 10μl.RESULTS:
The linear range was 34.8-139.4μg/ml for chloramphenicol(r=0.999 8)and 93.0-930.5μg/ml for diphenhydramine hydrochloride (r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 97.70%-104.02%(RSD=2.2%,n=9)and 96.72%-101.72%(RSD=1.6%,n=9).CONCLUSIONS:
The method is specific,accurate with good precision,and suitable for the quality control of Chlorobenzene alcohol solution.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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