Optimization of the Extraction Technology of Nucleosides from Codonopsis pilosula by Orthogonal Test / 中国药房
China Pharmacy
;
(12): 2254-2256, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504487
ABSTRACT
OBJECTIVE:
To optimize the extraction technology of nucleoside from Codonopsis pilosula.METHODS:
The ultra-sonic extraction was used to extract nucleoside from C. pilosula. The extraction technology was screened and optimized by single factor and orthogonal test with ethanol concentration (including water),extraction time,solid-liquid ratio and extraction times as factors using the total contents of cytidine,uridine,guanosine and adenosine as index. Validation test was conducted.RESULTS:
The optimal extraction technology was that pure water was extraction solvent,extracting for 45 min,with material-liquid ratio of 1∶20 (g/ml),extracting for 2 times. Total amount of 4 kinds of nucleosides from in 100 g C. pilosula was (17.01 ± 0.005) mg (RSD=0.68%,n=3).CONCLUSIONS:
Optimized extraction technology of nucleoside from C. pilosula is stable,feasible,rapid in operation and cost-saving.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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