IAP-1 gene silencing enhanced the radiosensitivity of liver cancer SMMC-7721 cells / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 130-133, 2017.
Article
in Zh
| WPRIM
| ID: wpr-506053
Responsible library:
WPRO
ABSTRACT
Objective To explore the effect of cellular inhibitor of apoptosis protein1 (cIAP-1) gene on the radiosensitivity of SMMC-7721 cells.Methods We silenced cIAP-1 expressions by the shRNA technology,and then we detected the changes of cell proliferation,cell cycle and cell apoptosis by CCK8 as-say,Western blot,qRT-PCR and flow cytometry after the radiotherapy.Results The cell proliferation rate of liver cancer SMMC-7721 cells at different radiation doses of 1 Gy,4 Gy,7 Gy and 10 Gy was detected.Comparing with control group (pGCsi-H1-control),the cell proliferation in cIAP-1 silencing group (pGCsiH1-shRNA) was significantly reduced at various radiation doses,and the effect was dose-dependent (P < 0.05).G1/G0 phase arrest was observed after radiation (P <0.01),and the proportion of cells in S phase was significantly reduced compared with control (P < 0.01).Compared with control group,G1/G0 phase arrest was detected (P < 0.05),and the percentage of cell apoptosis was increased significantly in cIAP-1 silencing group (P < 0.05).Conclusion cIAP-1 silencing can enhance the radiosensitivity of liver cancer cells,and inhibit cell proliferation by promoting the anti-tumor effect of radiation.
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WPRIM
Language:
Zh
Journal:
Chinese Journal of Hepatobiliary Surgery
Year:
2017
Type:
Article